【測定実施例】

• 培養細胞中の微量金属の測定
• 植物根部および土壌中の微量金属測定
• 破砕菌体（イースト）中のZnイオン定量

【その他参考情報】

• 試料の基本調整法
• アッセイのコツ（撹拌)

【本法使用文献】

• Kamei S et al.
  Zinc Deficiency via a Splice Switch in Zinc Importer ZIP2/SLC39A2 Causes Cystic Fibrosis-Associated MUC5AC Hypersecretion in Airway Epithelial Cells.
  EBioMedicine. 2017 Dec 25.
• Tanaka K et al.
  Thioredoxin-albumin fusion protein prevents copper enhanced zinc-induced neurotoxicity via its antioxidative activity.
  Int J Pharm. 2017 Nov. PubMed PMID: 29122608.
• Ratanayotha A et al.
  Molecular and functional characterization of the voltage-gated proton channel in zebrafish neutrophils.
  Physiol Rep. 2017 Aug. PubMed PMID: 28774948.
• Orino K.
  Inhibitory effect of transferrin on serum zinc measurement.
  Biometals. 2017 Aug. PubMed PMID: 28677078.
  
• Narukawa M et al.
  Participation of the peripheral taste system in aging-dependent changes in taste sensitivity.
  Neuroscience. 2017 Jul 4. PubMed PMID: 28687314.
  
• Mochizuki A et al.
  Cytocompatibility of magnesium and AZ31 alloy with three types of cell lines using a direct in vitro method.
  J Mater Sci Mater Med. 2016 Sep.PubMed PMID: 27568216.
• Hattori Y et al.
  Metallothionein MT2A A-5G Polymorphism as a Risk Factor for Chronic Kidney Disease and Diabetes: Cross-Sectional and Cohort Studies.
  Toxicol Sci. 2016 Jul. PubMed PMID: 27122239.
• Oboshi M et al.
  Attenuation of hypertension and renal damage in renovascular hypertensive rats by iron restriction.
  Hypertens Res. 2016 Dec. PubMed PMID: 27439494.
• Suzuki T et al.
  Dietary zinc deficiency induces oxidative stress and promotes tumor necrosis factor-α- and interleukin-1β-induced RANKL expression in rat bone.
  J Clin Biochem Nutr. 2016 Mar. PubMed PMID: 27013778.
• Furukawa Y et al.
  Conformational Disorder of the Most Immature Cu, Zn-Superoxide Dismutase Leading to Amyotrophic Lateral Sclerosis.
  J Biol Chem. 2016 Feb. PubMed PMID: 26694608.
• Kuribayashi T et al.
  Simple differentiation of sake (Japanese alcoholic beverage) based on trace inorganic components using colorimetric methods.
  J Biosci Bioeng. 2016 Jan. PubMed PMID: 26077711.
  
• Suzuki T et al.
  Zinc Deficiency Increases Serum Concentrations of Parathyroid Hormone through a Decrease in Serum Calcium and Induces Bone Fragility in Rats.
  J Nutr Sci Vitaminol (Tokyo). 2015. PubMed PMID: 26639846.
• Suzuki T et al.
  Dietary zinc supplementation increased TNFα and IL1β-induced RANKL expression, resulting in a decrease in bone mineral density in rats.
  J Clin Biochem Nutr. 2016 Jan. PubMed PMID: 26798197.
• Hanagata N et al.
  Calcium ions rescue human lung epithelial cells from the toxicity of zinc oxide nano particles.
  J Toxicol Sci. 2015. PubMed PMID: 26354379.
• Fujihara J et al.
  Distribution and toxicity evaluation of ZnO dispersion nanoparticles in single intravenously exposed mice.
  J Med Invest. 2015. PubMed PMID: 25817283.
• Okada A et al.
  Analysis of the binding of bovine and human fibrinogen to ferritin: evidence that fibrinogen is a common ferritin-binding protein in mammals.
  Biometals. 2015 Aug. PubMed PMID: 25860295.
• Mizuno D et al.
  Protective activity of carnosine and anserine against zinc-induced neurotoxicity: a possible treatment for vascular dementia.
  Metallomics. 2015 Aug. PubMed PMID: 25846004.
• Ohtsuka H et al.
  Sexual development of Schizosaccharomyces pombe is induced by zinc or iron limitation through Ecl1 family genes.
  Mol Genet Genomics. 2015 Feb. PubMed PMID: 25204792.
• Nakanishi K et al.
  Synthesis of amino-functionalized mesoporous silica sheets and their application for metal ion capture.
  Journal of Asian Ceramic Societies. 2014 Nov.
• Mochizuki A et al.
  Study on the blood compatibility and biodegradation properties of magnesium alloys.
  Mater Sci Eng C Mater Biol Appl. 2015 Feb. PubMed PMID: 25492190.
• Sakurai Y et al.
  A primary role for disulfide formation in the productive folding of prokaryotic Cu,Zn-superoxide dismutase.
  J Biol Chem. 2014 Jul. PubMed PMID: 24917671.
• Minami A et al.
  Improvement of depression-like behavior and memory impairment with the ethanol extract of Pleurotus eryngii in ovariectomized rats.
  Biol Pharm Bull. 2013. PubMed PMID: 24292057.
  
• Homma K et al.
  SOD1 as a molecular switch for initiating the homeostatic ER stress response under zinc deficiency.
  Mol Cell. 2013 Oct. PubMed PMID: 24076220.
• Isobe N et al.
  TEMPO-oxidized cellulose hydrogel as a high-capacity and reusable heavy metal ion adsorbent.
  J Hazard Mater. 2013 Sep. PubMed PMID: 23747479.
• Chou J et al.
  The therapeutic effect on bone mineral formation from biomimetic zinc containing tricalcium phosphate (ZnTCP) in zinc-deficient osteoporotic mice.
  PLoS One. 2013 Aug. PubMed PMID: 23967249.
• Zhuang F et al.
  Synergic Toxicity of Solid Particles and Released Zinc from Zinc Oxide Nanoparticles to Human Lung Epithelial Cells.
  Nano Biomedicine. 2012.

【本キット参考文献（論文記載用）】

• Makino, T., Saito,M., Horiguchi, D. and Kina, K. :A highly sensitive colorimetric determination of serum zinc using water-soluble pyridylazo dye. Clin. Chim. Acta, 120, 127－135（1982）
• → 製品情報はこちら（メタロアッセイ 亜鉛測定LS）

【測定実施例】

• 唾液検体の調整例

【その他参考情報】

• 試料の基本調整法
• アッセイのコツ（撹拌)

【本キット参考文献（論文記載用）】

• Makino, T., Saito,M., Horiguchi, D. and Kina, K. :A highly sensitive colorimetric determination of serum zinc using water-soluble pyridylazo dye. Clin. Chim. Acta, 120, 127－135（1982）
• → 製品情報はこちら（メタロアッセイ 低濃度亜鉛測定LS）

【その他参考情報】

• 試料の基本調整法
• アッセイのコツ（撹拌)

【本法使用文献】

　　　　　　　　　　　　　　　　　　　　　　
• Otsuka M et al.
  Therapeutic effects of transdermal systems containing zinc-related materials on thermal burn rats.
  Biomed Mater Eng. 2015. PubMed PMID: 25813953.

　　　　　　　　　　　　　　　　　　　　　　

【本キット参考文献（論文記載用）】

• Makino, T., Saito,M., Horiguchi, D. and Kina, K. :A highly sensitive colorimetric determination of serum zinc using water-soluble pyridylazo dye. Clin. Chim. Acta, 120, 127－135（1982）